DRO-GRO-TPH Groundwater plume and soil Endophyte assisted Phytoremediation site CA Central Valley

Phase A: Engineering firm attempted phytoremediation using un-inoculated poplar trees. Tree survival rate ~30%. Intrinsyx was brought on 1 year into the project and performed an endophyte root drench on the poplars. Endophyte inoculated poplars had a 98% survival rate.


Phase B: Intrinsyx worked with firm on Phase B planting of hybrid poplars. Inoculated trees had a 98% survival rate and gained hydrological control of the water table.


Phase B planting


5 months after planting

Over 1,000 hybrid poplar and willows were planted in total. The projects success has allowed for the shutoff of SVE (1Q2019) and GWP&T (1Q2020) systems.

This project was deployed for one of the world’s largest oil and gas companies, on their property in central California at a former petroleum transfer terminal with high levels of diesel and gas in groundwater and with high soil concentrations. In spring of 2017 we were brought in after a tree die-off event to advise the client on changes to make going forward which included a survey of past methods used, inoculation and demonstration of the benefits of our PD1 bacterial tree endophyte strain. Our goal was to recover a portion of the still alive, but unhealthy trees, through the inoculation of a subset of existing trees using our PD1 endophyte. The remaining live adjacent unhealthy trees were used as immediate controls for comparison.


We inoculated a subset of existing but unhealthy trees by pouring our PD1 endophyte culture broth around the base of the trees. We also made recommendations on proper tree care techniques to help all trees (inoculated and any uninoculated trees) survive during this study. After a couple of months, the trees were surveyed again for growth characteristics and chlorophyll content. Remarkably, the trees treated with our PD1 endophytes exhibited 100% greater growth, demonstrated extensive new shoot growth, and a 25% to 100% greater chlorophyll content when compared to the untreated controls.  The client insisted on working with Intrinsyx as a consultant and provider of the endophyte and salt/B tolerant poplar and willow trees. The system was duplicated throughout the site which now has 1,400 healthy alive large trees. The trees provided complete hydrological control on the site within four years. Planting of these trees has allowed for site groundwater hydrological control, and regulator approved discontinuation of costly groundwater pump and treat and soil vapor extraction systems. The natural source zone depletion rates in the tree plantations are very high vs low levels off tree site in soil alone.




Central California

Contaminated Mediums:

Groundwater, Soil

Type of Remediation:

Endophyte Assisted Phytoremediation

Services Provided: 

Site Troubleshooting and Maintenance, Biological Monitoring